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KMID : 1007520020110060694
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Food Science and Biotechnology
2002 Volume.11 No. 6 p.694 ~ p.696
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Effect of γ-irradiation on the Molecular Properties of Mushroom Polyphenoloxidase
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Sun Namkyu
Song Kyung-Bin
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Abstract
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To elucidate the inactivation mechanism of mushroom polyphenoloxidasde by γ-irradiation, enzyme solutions were irradiated at 1,3,5,7, and 10 kGy, and molecular properties such as secondary and tertiary structures were examined. Irradiation above 5kGy decreased the enzyme activity by more than 93%. Circular dichroism study showed that increase of radiation increased the ellipticity values in the rage of 210 and 225 nm, resulting in decrease of the ordered structure of the enzyme. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm, indicating changes in the tertiary structure of the enzyme. This study clearly shows that γ-irradiation 10kGy can significantly alter the molecular properties and inactivation of the enzyme.
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